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Hybridization of DNA to bead-immobilized probes c...
Replication of DNA microarrays from zip code maste...
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Transfer of surface polymerase reaction products to a secondary platform with conservation of spatial registration
| Journal | J. Am. Chem. Soc. |
|---|---|
| Author | Kim, J.; Crooks, R. M. |
| Citation | J. Am. Chem. Soc., 2006, 128, 12076-12077 |
| DOI | https://doi.org/10.1021/ja0646139 |
Here, we describe a method for directly transferring very small amounts of reaction products from one surface to another. The approach is illustrated using a T4 DNA polymerase reaction to extend primers hybridized to a surface-confined DNA template. Following the extension reaction, the resulting oligonucleotide is transferred to a product surface. The important results are that (1) the spatial registration of the product is preserved after transfer; (2) the same reactant surface can be used to generate and transfer multiple iterations of products; and (3) the reaction products are biologically active after transfer.
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Hybridization of DNA to bead-immobilized probes confined within a microfluidic channel
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Transfer of surface polymerase reaction products to a secondary platform with conservation of spatial registration
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Replication of DNA microarrays from zip code masters
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Parallel fabrication of RNA microarrays by mechanical transfer from a DNA master
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Replication of DNA microarrays prepared by in situ oligonucleotide polymerization and mechanical transfer.
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