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Hybridization of DNA to bead-immobilized probes confined within a microfluidic channel
| Journal | Langmuir |
|---|---|
| Author | Kim, J.; Heo, J.; Crooks, R. M. |
| Citation | Langmuir, 2006, 22, 10130-10134 |
| DOI | https://doi.org/10.1021/la0616956 |
We report the factors influencing the capture of DNA by DNA-modified microbeads confined within a microfluidic channel. Quantitative correlation of target capture efficiency to probe surface concentration, solution flow rate, and target concentration are discussed. The results indicate that the microfluidic system exhibits a limit of detection of 
10-10 M (10-16mol) DNA and a selectivity factor of 8 × 103. Typical hybridization times are on the order of minutes.
Transfer of surface polymerase reaction products to ...
| 제목 | Author | Journal | Citation |
|---|---|---|---|
Hybridization of DNA to bead-immobilized probes confined within a microfluidic channel
|
Kim, J.; Heo, J.; Crooks, R. M. | Langmuir | Langmuir, 2006, 22, 10130-10134 |
|
Transfer of surface polymerase reaction products to a secondary platform with conservation of spatial registration
|
Kim, J.; Crooks, R. M. | J. Am. Chem. Soc. | J. Am. Chem. Soc., 2006, 128, 12076-12077 |
|
Replication of DNA microarrays from zip code masters
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Lin, H.; Kim, J.; Sun, L.; Crooks, R. M. | J. Am. Chem. Soc. | J. Am. Chem. Soc., 2006, 128, 3268-3272 |
|
Parallel fabrication of RNA microarrays by mechanical transfer from a DNA master
|
Kim, J.; Crooks, R. M. | Anal. Chem. | Anal. Chem., 2007, 79, 8994-8999 |
|
Replication of DNA microarrays prepared by in situ oligonucleotide polymerization and mechanical transfer.
|
Kim, J.; Crooks, R. M. | Anal. Chem. | Anal. Chem., 2007, 79, 7267-7274 |
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