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Hybridization of DNA to bead-immobilized probes confined within a microfluidic channel

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Journal Langmuir
Author Kim, J.; Heo, J.; Crooks, R. M.
Citation Langmuir, 2006, 22, 10130-10134
DOI https://doi.org/10.1021/la0616956


We report the factors influencing the capture of DNA by DNA-modified microbeads confined within a microfluidic channel. Quantitative correlation of target capture efficiency to probe surface concentration, solution flow rate, and target concentration are discussed. The results indicate that the microfluidic system exhibits a limit of detection of 10-10 M (10-16mol) DNA and a selectivity factor of 8 × 103. Typical hybridization times are on the order of minutes.


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